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The first three measurements were used to establish a baseline rate

Then, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:200 dilution, BOSTER, Cat.# BA1054) for 2 h at room temperature

The level of dietary protein affects the vitamin B-6 requirement of cats

Ethics approval and consent to participate All animal experiments within this investigation adhered strongly to the guidelines outlined by the National Institutes of Health for the Care and Use of Laboratory Animals (NIH Publication No